Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: Summary of the Percoll gradient centrifugation procedure developed to separate bacterial subpopulation with time estimate for each step

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Gradient Centrifugation

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: Percoll discontinuous density gradient profile for the fractionation of SCF and NCF from M. smegmatis ( Msm ) and M. tuberculosis ( Mtb ) cultures.

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Fractionation, Centrifugation

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: CFU determination for the number of cells present in each fraction of SCF and NCF from M. smegmatis (Msm) and M. tuberculosis (Mtb) cultures.

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques:

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: Percoll discontinuous density gradient centrifugation-based fractionation of SCs and NCs from Mycobacterium smegmatis cultures. ( A ) Fractionation steps and the extent of enrichment of NCs and SCs. ( B ) The ultracentrifuge tube after fractionation.

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Gradient Centrifugation, Fractionation

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: DIC images of SCF1, SCF2 and NCF from Msm cultures after Percoll discontinuous density gradient fractionation. Scale, 5 µm.

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Fractionation

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet: Percoll discontinuous density gradient centrifugation-based fractionation of SCs and NCs from Mycobacterium tuberculosis cultures. Cartoon showing the fractionation steps and the extent of enrichment of NCs and SCs. ND – Very few or no cells.

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Gradient Centrifugation, Fractionation

Journal: MethodsX

Article Title: Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

doi: 10.1016/j.mex.2023.102344

Figure Lengend Snippet:

Article Snippet: In addition, M. smegmatis SCF and NCF cells (∼ 3 × 10 6 cells/ml) obtained from the Preparative Scale Percoll discontinuous density gradient centrifugation were inoculated at 1% inoculum into fresh Middlebrook 7H9 medium.

Techniques: Gradient Centrifugation, Sterility, Saline, Fractionation, Microscopy

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Recipient HO-1 Inducibility is Essential for Post-Transplant Hepatic HO-1 Expression and Graft Protection: From Bench-to-Bedside

doi: 10.1111/ajt.15043

Figure Lengend Snippet: Casein-elicited peritoneal neutrophils were obtained from HO-1 proficient control and myeloid-specific HO-1 knockout (mHO-1 KO) mice. (A) ROS level in neutrophil was analyzed by flow cytometry using fluorescent CellROX probe (n=4/group). (B) qRT-PCR-assisted detection of mRNA coding for HO-1, TNFα, CXCL1, CXCL2 and CXCL10. Data were normalized to β-actin gene (n=4/group). Data shown as mean±SD (*p<0.05, Student t-test).

Article Snippet: Casein-elicited peritoneal neutrophils were purified by Percoll density gradient centrifugation (Neutrophil Isolation Kit, Cayman Chemical, Ann Arbor, MI) ( 26 ).

Techniques: Control, Knock-Out, Flow Cytometry, Quantitative RT-PCR